Journal: Heliyon

Article Title: Erzhi pills reverse PD-L1-mediated immunosuppression in melanoma microenvironment

doi: 10.1016/j.heliyon.2024.e24988

Figure Lengend Snippet: EZP increased the percentage of CD4 + T cells and enhanced the function of CD8 + T in vivo . (A–D) T cells were analyzed by flow cytometry. (A) The gate strategy for CD4 + and CD8 + T cells. Flow cytometry plots (B) and bar graphs present percentages of CD3 + CD4 + T cells (C) and CD3 + CD8 + T cells (D) in the spleen from each group mice. n = 5. (E–H) IHC staining for the tumor tissues. IHC images (E) and quantifications of CD4 + T cells (F), CD8 + T cells (G) and GZMB + cells (H) in tumor tissues. Scale bar, 100 μm. n = 3. Data are expressed as mean ± SD. * P < 0.05, ** P < 0.01 and *** P < 0.001 vs DMSO. IHC, immunohistochemistry; GZMB, granzyme B.

Article Snippet: PerCP/Cyanine5.5 anti-mouse CD3 antibody (E-AB-F1013J), FITC anti-mouse CD8a antibody (E-AB-F1104C) and PE anti-mouse CD4 antibody (E-AB-F1097D) were obtained from Elabscience (Wuhan, China).

Techniques: In Vivo, Flow Cytometry, Immunohistochemistry

Journal: PLoS ONE

Article Title: Modified vaccinia Ankara expressing EEHV1A glycoprotein B elicits humoral and cell-mediated immune responses in mice

doi: 10.1371/journal.pone.0265424

Figure Lengend Snippet: Splenic T cells from immunized mice were assessed for cytokine production after stimulation with gB peptide pools. Blue circles represent splenic T cells from animals immunized with MVA-gBΔfur731 and gray squares represent mice immunized with wtMVA. ( A ) Percentage of CD4 and CD8 cells producing at least one of three cytokines IFNγ, TNFα, or IL-2. ( B ) Percentage of CD4 cells producing at least one of three cytokines IFNγ, TNFα, or IL-2. ( C ) Percentage of CD8 cells producing at least one of three cytokines IFNγ, TNFα, or IL-2. Median values ± SD values for each group are shown as horizontal lines and error bars. Asterisks (*/**/****) indicate statistically significant differences ( p < 0.05/ p < 0.01/ p < 0.0001) between stimulation groups or MVA-gBΔfur731 and MVA immunized groups, as determined by two-way ANOVA with Tukey’s multiple comparisons test. A p value < 0.05 denotes significance.

Article Snippet: CD8a , FITC , 1:800 , Tonbo , 35-0081-U500 , 53–6.7.

Techniques:

Journal: PLoS ONE

Article Title: Modified vaccinia Ankara expressing EEHV1A glycoprotein B elicits humoral and cell-mediated immune responses in mice

doi: 10.1371/journal.pone.0265424

Figure Lengend Snippet: CD8 + T cells positive for single cytokine activation markers IFNγ ( A ), TNFα ( B ), or IL-2 ( C ), or double positive cytokine activation markers IFNγ/TNFα ( D ), IL-2/TNFα ( E ), or IFNγ/IL-2 ( F ). CD8 + T cells isolated from splenocytes of immunized mice were assessed for cytokine production after gB peptide pool stimulation. Blue circles represent splenic T cells from animals immunized with MVA-gBΔfur731 and gray squares represent mice immunized with wtMVA. Median values ± SD values for each group are shown as horizontal lines and error bars. Asterisks (*/**/***/****) indicate statistically significant differences ( p < 0.05/ p < 0.01/ p < 0.001/ p < 0.0001) between stimulation groups or MVA-gBΔfur731 and wtMVA immunized groups, as determined by two-way ANOVA with Tukey’s multiple comparisons test. A p value < 0.05 denotes significance.

Article Snippet: CD8a , FITC , 1:800 , Tonbo , 35-0081-U500 , 53–6.7.

Techniques: Activation Assay, Isolation